Association between α4 integrin cytoplasmic tail and non-muscle myosin IIA regulates cell migration

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Association between α4 integrin cytoplasmic tail and non-muscle myosin IIA regulates cell migration.

α4β1 integrin regulates cell migration via cytoplasmic interactions. Here, we report an association between the cytoplasmic tail of α4 integrin (α4 tail) and non-muscle myosin IIA (MIIA), demonstrated by co-immunoprecipitation of the MIIA heavy chain (HC) with anti-α4-integrin antibodies and pull-down of MIIA-HC with recombinant α4 tail from cell lysates. The association between the α4 tail and...

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Non-muscle myosin IIA transports a Golgi glycosyltransferase to the endoplasmic reticulum by binding to its cytoplasmic tail.

The mechanism of the Golgi-to-ER transport of Golgi glycosyltransferases is not clear. We utilize a cell line expressing the core 2 N-acetylglucosaminyltransferase-M (C2GnT-M) tagged with c-Myc to explore this mechanism. By immunoprecipitation using anti-c-Myc antibodies coupled with proteomics analysis, we have identified several proteins including non-muscle myosin IIA (NMIIA), heat shock pro...

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Integrin Cytoplasmic Tail Interactions

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ژورنال

عنوان ژورنال: Journal of Cell Science

سال: 2011

ISSN: 1477-9137,0021-9533

DOI: 10.1242/jcs.074211